Microfluidic Assay for Continuous Bacteria Detection Using Antimicrobial Peptides and Isotachophoresis
نویسندگان
چکیده
منابع مشابه
Electrical detection of pathogenic bacteria via immobilized antimicrobial peptides.
The development of a robust and portable biosensor for the detection of pathogenic bacteria could impact areas ranging from water-quality monitoring to testing of pharmaceutical products for bacterial contamination. Of particular interest are detectors that combine the natural specificity of biological recognition with sensitive, label-free sensors providing electronic readout. Evolution has ta...
متن کاملAntimicrobial Peptides Targeting Gram-Positive Bacteria
Antimicrobial peptides (AMPs) have remarkably different structures as well as biological activity profiles, whereupon most of these peptides are supposed to kill bacteria via membrane damage. In order to understand their molecular mechanism and target cell specificity for Gram-positive bacteria, it is essential to consider the architecture of their cell envelopes. Before AMPs can interact with ...
متن کاملMicrofluidic aqueous two-phase system for continuous partitioning of bacteria
The interaction of bacterial cells with surrounding environment depends on its surface characteristics such as hydrophobicity, hydrophilicity balance and net charge. In this paper, aqueous two-phase system partitioning of Escherichia coli strains based on their difference in surface properties is introduced in a microfluidic system. While aqueous two-phase system is widely use to separate biomo...
متن کاملBead diffusion assay for discovering antimicrobial cyclic peptides.
A combinatorial library of 15,536 cyclic decapeptide analogues of tyrocidine A and gramicidin S was prepared on photocleavable TentaGel beads. The beads were photolyzed without solvent, and spread onto an agar plate inoculated with bacterial lawn. Clear zones were formed around beads carrying antimicrobially active peptides such as E18 c(kVOrnLfThiYOrnLq), which inhibited growth of B. subtilis ...
متن کاملTandem isotachophoresis-zone electrophoresis via base-mediated destacking for increased detection sensitivity in microfluidic systems.
Electrophoresis in microfluidic devices is becoming a useful analytical platform for a variety of biological assays. In this report, we present a method that allows for an increased sensitivity of detection of fluorescent molecules in microfluidic electrophoresis devices. This capability is provided by the implementation of a particular buffer system that is designed to initially function in an...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2014
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac5017776